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rox reference dye  (Jena Bioscience)


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    Structured Review

    Jena Bioscience rox reference dye
    Rox Reference Dye, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rox+reference+dye/ROX+Reference+Dye+-+25+%C2%B5M/pmc12890628-78-19-22
    Average 90 stars, based on 7 article reviews
    rox reference dye - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Comparative genomics of Fusarium oxysporum f. sp. melonis strains reveals nine lineages and a new sequence type of AvrFom2
    Article Snippet: Primers for quantitative real‐time PCR (qPCR) were designed using Primer3 software (http://primer3.ut.ee/ ) and are listed in Table . qPCR was carried out on a QuantStudio 3 Real‐Time PCR system (Thermo Fisher Scientific) with the following two‐step program: 95°C for 2 min; 40 cycles (95°C for 15 s, 62°C for 1 min) and a melting curve step at the end. .. All reactions were performed in a total volume of 10 μl containing 1 μl of 10× Super Taq buffer, 0.2 μl of dNTPs (10 mM each), 1 μl of each primer (3 pmol μl −1 ), 0.05 μl of Super Taq polymerase (SphaeroQ), 0.02 μl of ROX reference dye (Jena Bioscience), 0.2 μl of Evagreen fluorescent DNA stain (Jena Bioscience), 5.53 μl sterile Milli‐Q and 1 μl of 16× diluted template DNA. ..

    Article Title: A point-of-care assay for genotyping women prior to ovarian stimulation with recombinant or human menopausal gonadotropins in assisted reproduction
    Article Snippet: The assay performance was evaluated in gradient temperatures and time intervals in a MyBlock Mini Dry Bath (Benchmark Scientific Inc, Sayreville, USA). .. In the validation fluorescence-based assay, 1 μL SYBR Green Fluorescent DNA stain (Jena Bioscience, Jena, Germany) and 1 μL ROX Reference Dye (Jena Bioscience) was added. .. The samples were run in a Bio-Rad CFX thermocycler (Agilent Technologies, California, United States) for 50 minutes and monitored every 30 seconds in the SYBR channel.

    Sterility:

    Article Title: Comparative genomics of Fusarium oxysporum f. sp. melonis strains reveals nine lineages and a new sequence type of AvrFom2
    Article Snippet: Primers for quantitative real‐time PCR (qPCR) were designed using Primer3 software (http://primer3.ut.ee/ ) and are listed in Table . qPCR was carried out on a QuantStudio 3 Real‐Time PCR system (Thermo Fisher Scientific) with the following two‐step program: 95°C for 2 min; 40 cycles (95°C for 15 s, 62°C for 1 min) and a melting curve step at the end. .. All reactions were performed in a total volume of 10 μl containing 1 μl of 10× Super Taq buffer, 0.2 μl of dNTPs (10 mM each), 1 μl of each primer (3 pmol μl −1 ), 0.05 μl of Super Taq polymerase (SphaeroQ), 0.02 μl of ROX reference dye (Jena Bioscience), 0.2 μl of Evagreen fluorescent DNA stain (Jena Bioscience), 5.53 μl sterile Milli‐Q and 1 μl of 16× diluted template DNA. ..

    Biomarker Discovery:

    Article Title: A point-of-care assay for genotyping women prior to ovarian stimulation with recombinant or human menopausal gonadotropins in assisted reproduction
    Article Snippet: The assay performance was evaluated in gradient temperatures and time intervals in a MyBlock Mini Dry Bath (Benchmark Scientific Inc, Sayreville, USA). .. In the validation fluorescence-based assay, 1 μL SYBR Green Fluorescent DNA stain (Jena Bioscience, Jena, Germany) and 1 μL ROX Reference Dye (Jena Bioscience) was added. .. The samples were run in a Bio-Rad CFX thermocycler (Agilent Technologies, California, United States) for 50 minutes and monitored every 30 seconds in the SYBR channel.

    Fluorescence:

    Article Title: A point-of-care assay for genotyping women prior to ovarian stimulation with recombinant or human menopausal gonadotropins in assisted reproduction
    Article Snippet: The assay performance was evaluated in gradient temperatures and time intervals in a MyBlock Mini Dry Bath (Benchmark Scientific Inc, Sayreville, USA). .. In the validation fluorescence-based assay, 1 μL SYBR Green Fluorescent DNA stain (Jena Bioscience, Jena, Germany) and 1 μL ROX Reference Dye (Jena Bioscience) was added. .. The samples were run in a Bio-Rad CFX thermocycler (Agilent Technologies, California, United States) for 50 minutes and monitored every 30 seconds in the SYBR channel.

    SYBR Green Assay:

    Article Title: A point-of-care assay for genotyping women prior to ovarian stimulation with recombinant or human menopausal gonadotropins in assisted reproduction
    Article Snippet: The assay performance was evaluated in gradient temperatures and time intervals in a MyBlock Mini Dry Bath (Benchmark Scientific Inc, Sayreville, USA). .. In the validation fluorescence-based assay, 1 μL SYBR Green Fluorescent DNA stain (Jena Bioscience, Jena, Germany) and 1 μL ROX Reference Dye (Jena Bioscience) was added. .. The samples were run in a Bio-Rad CFX thermocycler (Agilent Technologies, California, United States) for 50 minutes and monitored every 30 seconds in the SYBR channel.

    Article Title: Molecular Characterization of the NLRC4 Expression in Relation to Interleukin-18 Levels
    Article Snippet: Briefly, RNA was purified using TRIzol (Life Technologies, Darmstadt, Germany) and reverse transcribed using High-Capacity cDNA Reverse Transcription Kit (Life Technologies, Darmstadt, Germany) according to manufacturers’ protocols. .. Real-time PCR was performed in a 10μl reaction volume using 5 μlL ABsolute qPCR SYBR Green Mix (Life DOI: 10.1161/CIRCGENETICS.115.001079 8 Technologies, Darmstadt, Germany), 0.2 μl ROX Reference Dye (Jena Bioscience, Oxford, England), 0.25 ng cDNA and 150 - 500 nM primers (Supplemental Table 3). ..

    Article Title: Molecular Characterization of the NLRC4 Expression in Relation to Interleukin-18 Levels
    Article Snippet: Briefly, RNA was purified using TRIzol (Life Technologies, Darmstadt, Germany) and reverse transcribed using High-Capacity cDNA Reverse Transcription Kit (Life Technologies, Darmstadt, Germany) according to manufacturers’ protocols. .. Real-time PCR was performed in a 10μl reaction volume using 5 μlL ABsolute qPCR SYBR Green Mix (Life Technologies, Darmstadt, Germany), 0.2 μl ROX Reference Dye (Jena Bioscience, Oxford, England), 0.25 ng cDNA and 150 – 500 nM primers ( Supplemental Table 3 ). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Typing of Chlamydia psittaci to monitor epidemiology of psittacosis and aid disease control in the Netherlands, 2008 to 2013.
    Article Snippet: .. After optimisation, the final reaction volume (25 μl) included 5 μl eluate, 12.5 μl (2x) Greenmaster qPCR mix with ROX reference dye, uracil-N-glycosylase (Jena biosciences, Jena, Germany) and 0.5 μM of each primer. ..

    Article Title: Molecular Characterization of the NLRC4 Expression in Relation to Interleukin-18 Levels
    Article Snippet: Briefly, RNA was purified using TRIzol (Life Technologies, Darmstadt, Germany) and reverse transcribed using High-Capacity cDNA Reverse Transcription Kit (Life Technologies, Darmstadt, Germany) according to manufacturers’ protocols. .. Real-time PCR was performed in a 10μl reaction volume using 5 μlL ABsolute qPCR SYBR Green Mix (Life DOI: 10.1161/CIRCGENETICS.115.001079 8 Technologies, Darmstadt, Germany), 0.2 μl ROX Reference Dye (Jena Bioscience, Oxford, England), 0.25 ng cDNA and 150 - 500 nM primers (Supplemental Table 3). ..

    Article Title: Verapamil and Rifampin Effect on P-Glycoprotein Expression in Hepatocellular Carcinoma
    Article Snippet: .. PCR reactions were performed totally in a total volume of 25 μL mix (qPCR probe master, Jena Bioscience, Germany) containing 2 μL of the cDNA template, 4 pmol of each primer, 2 pmol of probes and 0.25 μL ROX reference dye. ..

    Article Title: Molecular Characterization of the NLRC4 Expression in Relation to Interleukin-18 Levels
    Article Snippet: Briefly, RNA was purified using TRIzol (Life Technologies, Darmstadt, Germany) and reverse transcribed using High-Capacity cDNA Reverse Transcription Kit (Life Technologies, Darmstadt, Germany) according to manufacturers’ protocols. .. Real-time PCR was performed in a 10μl reaction volume using 5 μlL ABsolute qPCR SYBR Green Mix (Life Technologies, Darmstadt, Germany), 0.2 μl ROX Reference Dye (Jena Bioscience, Oxford, England), 0.25 ng cDNA and 150 – 500 nM primers ( Supplemental Table 3 ). ..

    Polymerase Chain Reaction:

    Article Title: Verapamil and Rifampin Effect on P-Glycoprotein Expression in Hepatocellular Carcinoma
    Article Snippet: .. PCR reactions were performed totally in a total volume of 25 μL mix (qPCR probe master, Jena Bioscience, Germany) containing 2 μL of the cDNA template, 4 pmol of each primer, 2 pmol of probes and 0.25 μL ROX reference dye. ..



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